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Mechanistic investigations in the field have traditionally focused on single components, such as oocyte mitochondrial dysfunction [11, 12], local microenvironmental alterations [13,14,15], or epigenetic abnormalities [16,17,18]

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Quantitative real-time PCR The differential expression of F41E6.5 ( gox-1 ), C31C9.2 ( ghpr-1 ), gcst-1 , mel-32 , and gcs-1 genes was measured using qRT-PCR

[DOI] [PMC free article] [PubMed] [Google Scholar] 13.Bisen A.C., Rawat P., Sharma G., Sanap S.N., Agrawal S., Kumar S., Kumar A., Choudhury A.D., Kamboj S., Narender T., et al

If you use alpha hydroxy acids or salicylic acid, apply those at a different time of day