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Description

For NIH-3T3 experiments, cells were grown in DMEM plus 10% fetal bovine serum (Invitrogen)

Because injectable NAD+ becomes bioavailable more rapidly, it may more quickly engage in cellular processes such as mitochondrial energy production, DNA repair and oxidative stress regulation

doi:10.1038/nrd1031

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Furthermore, studies have established HNE cells as a reliable and less invasive surrogate for HBE cells for these functional studies (Brewington et al., 2018)