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Nociceptin opioid receptor (NOP) as a therapeutic target: Progress in translation from preclinical research to clinical utility

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Detection of S-glutathionylated FABP5 Cells were lysed in NP-40 lysis buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% Nonidet P-40, 1 mM EDTA] containing 20 mM N -ethylmaleimide (Sigma-Aldrich, E1271) and protease inhibitor cocktail (Roche, 04693132001)