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The gene CYP19A1 , identified in an OCT perturb-seq screen, was associated with increased tumor PD-L1 levels, potentially through concomitant decrease of TGF- in the TME and upregulation of activating receptor NKG2D on NK cells during CYP19A1 inhibiton [40, 122, 124]

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We outline the progression from static attenuation to dynamic, rational design, where programmable genetic circuits (e.g., synchronized lysis systems and hypoxia-responsive logic gates) enable bacteria to be engineered as intelligent microrobots that can be used for the safe delivery of therapeutics while inducing precise, on-target immune stimulation

In 2013, a study investigated the combination therapy of anti-Her-2 CD8 + T cells and anti-PD-1 antibody in murine breast cancer models