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However, directly applying the current standardized protocol of the system to clinically important pathogens with much longer growth cycles, such as Mycobacterium tuberculosis (which can take days or even weeks), presents challenges

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Briefly, an extract (1000 L) or standard solution (Gallic acid) was added to Folin-Ciocalteu reagent (5 mL) and allowed to react for 5 min at room temperature