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Cells were lyzed using lysis buffer (10% glycerol 1% triton, 150 mM NaCl, 10 mM EDTA 0.5 M, 20 mM Hepes 1M pH-7.4), followed by immunoprecipitation using FLAG-Sepharose beads and western blots analysis using GPX4 antibody

The endotoxin level is minimal, Elamipretide Protein was purified using conventional chromatography techniques