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Before biotinstreptavidin pulldown, magnetic streptavidin beads (Thermo Fisher Scientific, 88817) were washed three times in 0.2% SDS in 50 mM HEPES

5-tryptophan hydroxylase is considered to be essential for the synthesis and release of serotonin, a peptide associated with neuroprotective and geroprotective properties

This allows controlled, repeated access from a single multi-dose vial within the validated use window

But there are features of lupus itself as well as the drugs used to treat lupus that significantly increase osteoporosis risk