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Essentially, AAV particles of the PHP.eB capsid (serotype), known to efficiently transduce the central nervous system via intravenous injection 42 , expressing Cre recombinase driven by the astrocyte-specific short GFAP promoter (PHP.eB-AAV-gfaABC 1 D-Cre-GFP) were administered intravenously (50 l aliquots of a phosphate-buffered saline (PBS) solution containing 0.001% Pluronic F-68, Sigma-Aldrich and 1 10 11 viral genomes, VG) through the retro-orbital sinus to 2-month-old Cpt1a lox/lox male mice under a brief sevoflurane anaesthesia (Sevorane, at 6% for initiation followed by roughly 3% for maintenance in air with supplements of O 2 and NO 2 0.4 and 0.8 l min 1 , respectively, using a gas distribution column, Hersill H-3, and a vaporizer, InterMed Penlons Sigma Delta)

When in doubt, err on the side of caution and use a new vial

After that, cells from the top of the chamber membrane were gently removed and the invasion cells on the bottom of the membrane were fixed with 4% paraformaldehyde and stained with 0.1% crystal violet (Solarbio)

Resveratrol modulates the gut microbiota and inflammation to protect against diabetic nephropathy in mice

Du Q, Zhang S, Li A, Mohammad IS, Liu B, Li Y