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Then, 0.1 mL of 1 M NaOH solution was introduced, and the mixture was mixed in the thermomixer at 900 rpm for 6 h at 60 C

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For example, the PC formation of AtPCS1 activated by Cd 2+ is usually 25 times more efficient than the PC synthesis rate measured in the presence of Cu +/2+ [4, 6, 53]