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Real-time PCR was performed using an S1000 PCR instrument (Bio-Rad, Hercules, CA, USA) to quantify gene expression

47,61 d,l -Leucovorin Improves Verbal Communication in Children With Autism Spectrum Disorder and Folate Receptor Alpha Autoantibodies Given the high prevalence of FRAAs in children with ASD and the effectiveness and safety of d,l -leucovorin, Drs Frye and Rossignol conducted a large prospective open-label case-series in which 44 children with ASD with a positive test for at least one FRAA were treated with 2 mg/kg/day (maximum 50 mg daily) of leucovorin in 2 divided doses

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