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I think the scale is unhealthy for me

Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

Looking down at an angle makes the meniscus appear higher or lower than it actually is Draw past your target by a few units, then push the plunger back to the exact mark