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It is considered experimental and should be approached with appropriate caution

Cells were fixed using 10% buffered formalin for 10 minutes, then permeabilized in blocking buffer (10% serum, 1% Triton X-100, in 1X PBS) for 1 hour, then primary antibody diluted in blocking buffer was applied overnight (See supplemental table 5 for antibody concentrations)

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We also demonstrated a promising approach to selectively inhibit the actions of undesirable GUSs

HTML is then cleaved by HTML aldolase (HTMLA, a pyridoxal phosphate requiring enzyme), yielding 4-trimethylaminobutyraldehyde (TMABA) and glycine