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GST pulldown assay Differentiated 3T3-L1 cells were lysed in immunoprecipitation (IP) lysis buffer (20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, and 1 mM EGTA, 2.5 mM sodium pyrophosphate, 1 mM -glycerophosphate, 1 mM Na 3 VO 4 , 1 mM PMSF, and 1X PIC)

Y., Ima-Nirwana, S., & Chin, A

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Particularly, Hg, Cd, and Pb levels exceeded the respective critical values 1.02, 0.30 and 0.25 g/wet weight (that is, the legal safety limits established by the European Community) in eight, three, and two of the swordfish specimens examined, respectively

The changes presented here directly influenced selenoprotein expression and host antioxidant metabolism