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The plate was placed into a 37 C incubator for 2 h before 2 l of a 5 M dBET6 solution in RPMI with 10% FBS was added to each well and the plate was again placed on an orbital shaker for 15 s (final concentration of dBET6: 500 nM)

Molecular and epigenetic mechanisms of Cr(VI)-induced carcinogenesis

dCas9 fuses with the catalytic domain of DNMT or TET proteins, and the CRISPR/DCas9-DNMT or DCas9-TET system enables precise editing of DNA methylation

(PubMed) Suh JH, Shenvi SV, Dixon BM, et al