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The sample size was determined based on a pre-experimental power analysis using data from a pilot study, which indicated that a minimum of n = 6 per group would provide 80% power to detect a 30% difference in primary outcomes at a significance level of = 0.05

Table S1)

For example, due to the downregulation of genes for purinergic receptors (e.g., P2ry12 ) and potassium leak channels (e.g., Thik-1 ) in aged mice, the ability for microglia to sense hyperactive neurons and migrate toward them in order to facilitate rapid repolarization would be reduced, impairing their beneficial modulation (Hickman et al., 2013