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Pyroglutamic acid (PCA), also known as 5-oxoproline, is a cyclic derivative of glutamic acid (or glutamine) and a gamma-glutamyl cycle metabolite indicative of glutathione turnover [149]

Measure of competitive binding titers by ELISA Coating, blocking, and gH/gL immobilization steps were performed as described under Measurement of serum antibody endpoint binding titers by anti-His capture ELISA. Following the capture of monomeric gH/gL, equal amounts of sera from each mouse in a group were pooled and diluted in blocking buffer and 2-fold serial dilutions were performed, followed by a 1 h incubation at 37 C

Contribution of increased glutathione content to mechanisms of oxidative stress resistance in hydrogen peroxide resistant hamster fibroblasts

Finally, minimum inhibitory concentrations (MIC) against silver acetate were determined using standard Clinical and Laboratory Institute (CLSI) broth-microdilution method

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