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Band on the pyruvate kinase muscle form 2 among the table 112:HepG2 2 times bands of pyruvate kinase muscle form (58KD) among the table 113:HepG2 Table 114: band on the pyruvate kinase muscle form 2 in the HASMC cell of processing back Lactic acid dehydrogenase (LDH) LDH is that NADH and NAD are followed in catalysis pyruvate and Lactated change simultaneously + The enzyme of change.It has under low cell oxygen tension acetone acid is changed into lactate under the situation of victim line plastochondria oxidative phosphorylation, to produce reducing equivalent and the ability that produces ATP.Cancerous cell shows that usually the expression of the LDH of increase flows and produce ATP and reducing equivalent and go back promitocondrion oxidative phosphorylation enzyme system (0XPHOS) to keep glycolysis.Therefore, the expression of LDH can make metabolism be transformed into the promotion pyruvate from Lactated generation and get into the TCA circulation in the minimizing cell.The lactic acid dehydrogenase (LDH) that utilizes the treatment of coenzyme Q10 to reduce cancerous cell is expressed and is minimum to normal impact cell, thereby supports the effect of coenzyme Q10 minimizing the transformation that causes cancerous cell bioenergetics to the mitochondrion oxidative phosphorylation enzyme system source that produces ATP from glycolysis through the conversion that makes Cytoplasm pyruvate to lactic acid.The result is summarized in the following table

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Chen T, Ma J, Liu Y, et al