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Cells were plated on sterile glass chamber slides and were exposed to MitoTracker Red (Molecular Probes) as we have described previously (79)

Scientific Interest in GHK-Cu Within peptide research environments, GHK-Cu is frequently examined in studies involving copper-dependent enzymes and peptide signaling

Eye area: 2% concentration is standard for the periorbital area due to thinner, more sensitive skin

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FIGURE 9 4 Discussion GHK and its bioactive form, GHK-Cu, have been demonstrated to be safe and effective in various cellular, tissue, and animal models ( In IBD, the expression levels of tight junction proteins such as ZO-1 and Occludin, is generally suppressed, leading to increased intestinal permeability, enhanced pathogen infiltration, and exacerbated inflammatory responses ( in vivo and in vitro experimental models revealed that GHK-Cu upregulates the expression level of Occludin and ZO-1, suggesting its potential role in promoting intestinal barrier repair