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10.1093/jn/121.5.745 11 BaldariS.Di RoccoG.ToiettaG

Cell suspension was plated onto poly-D,L-ornithine-coated plates with DMEM, 1ITS (Life Technologies), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.5% FBS and 0.05% gentamicin (Sigma), 10 ng/ml PDGF and 10 ng/ml FGF (Peprotech), with full medium change the next day and half medium change every other day

The incidence of dementia among DS patients is 10% in the age range 3550 yr and 55% in the age range 5060 yr and becomes 75% above the age of 60 yr, but AD neuropathology is present in virtually all adults with DS older than 40 yr (460)