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Next, we further excluded low-quality cells with more than 25% mitochondrial reads, fewer than 100 or more than 3,000 expressed genes, or more than 5,000 UMI counts

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doi: 10.1146/annurev-physiol-021014-071931 74 KobayashiKSalathMPrattMMCartagenaNJSoloniFSeyboldZVet al

This modification results in dramatically different properties

The validity of existing animal models in accurately representing the complex pathophysiology of human PCOS is also a point of contention, which hampers their applicability to real-world scenarios