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Similar content being viewed by others Introduction Biopharmaceuticals, such as vaccines, heparin, insulin, growth hormone, hematopoietic growth factors, interferons, monoclonal antibodies, etc., are generally incompatible with oral delivery

Following fixation with 4% paraformaldehyde for 30-60 min and nuclear staining with 0.02% DAPI (Sigma-Aldrich, #D9542) for 10 min, slices were scanned in a 20-fold magnification by using a Zeiss Axiovert 200M microscope equipped with Hamamatsu Orca Flash 4.0 camera and TissueFAXS scanning software (TissueGnostics, Vienna, Austria)

The activity was followed by measuring the quantity of radioactive diol formed in the aqueous phase using a scintillation counter (TriCarb 2810 TR, Perkin Elmer, Shelton, CT)