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After the washing step, TMB substrate was added and kept at 37 C or 10 min in the dark to allow the color to develop

extorquens CM4 FolD activity had only been assessed so far with NADP + (Marx and Lidstrom, metF2 and folD are two of the most differentially expressed genes in the CM transcriptome (Table S5)

Illustrative of these biological activities includes the fragments ability to bind to at least one of the same antibodies which bind to the full-length protein, the fragments ability to interact with at lease one of the same proteins which bind to the full-length, the fragments ability to elicit at least one of the same immune responses as the full-length protein (i.e., to cause the immune system to create antibodies specific to the same epitope, etc.), the fragments ability to bind to at least one of the same polynucleotides as the full-length protein, the fragments ability to bind to a receptor of the full-length protein, the fragments ability to bind to a ligand of the full-length protein, and the fragments ability to multimerize with the full-length protein

A minor pathway involves oxidation of acetaminophen by cytochrome P450 enzymes (CYP1A2, CYP2E1, and CYP3A isozymes) to form a potentially toxic intermediate, N-acetyl-benzoquinoneimine