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This protein was labeled using a His-Tag Labeling Kit RED-tris-NTA (2nd Generation, NanoTemper) and kept constant at 10 nM

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These effects are mediated through the activation of autophagy-related proteins, such as microtubule-associated protein 1A/1B-light chain 3 (LC3) and Beclin-1 (BECN1), and the reduction of pro-inflammatory markers such as IL-1 and TNF- (Reference Xu, Li and Dai23)