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to an OD 600 of 0.7-0.8 ( 2-3 h) before induction with 0.5 mM IPTG and incubated for an additional 3 h

The proteins responsible for control of ROS levels include various isoforms of SOD, catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase-like (GPX-like), glutathione reductase (GR), thioredoxins, glutaredoxins, and peroxiredoxins (Fig

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The insertion of selenocysteine into selenoproteins during translation is directed by the presence of a selenocysteine-insertion sequence (SECIS) within selenoprotein mRNAs

Kruse T, Christoffersen B, Mortensen K, et al