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For qPCR analysis, 5 L of immunoprecipitated DNA was used as a template in a 10 L total reaction volume using TaqMan Advanced PCR Master Mix (Thermo Fisher Scientific) and target-specific TaqMan probes, prepared in 384-well plates

Nature (1990) 348(6302):63942

Additionally, many emphasize that the best Snap 8 serum is one stored in airless containers and kept refrigerated to maintain peptide stability and effectiveness over time