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The use the peptide Ac-Nle-c c/o(-Asp-His-D-Phe-Arg-Trp-Lys)-OH in the manufacture of a medicament for intranasal administration for use in the treatment of erectile dysfunction in a male, wherein the peptide Ac-Nle-cyc/o(-Asp-His-D-Phe-Arg-Trp-Lys)-OH increases cGMP levels to an amount sufficient to produce an erection sufficient for sexual intercourse when the peptide Ac-Nle-cyc/o(-Asp-His-D-Phe-Arg-Trp-Lys)-OH is administered at a sub-therapeutic dose and administered subsequent to a sub-therapeutic dose of a PDE-5 inhibitor

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Cell 166:13081323.e1330 Shin J, Berg DA, Zhu Y, Shin JY, Song J, Bonaguidi MA, Enikolopov G, Nauen DW, Christian KM, Ming GL et al (2015) Single-cell RNA-Seq with waterfall reveals molecular cascades underlying adult neurogenesis

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A small strip of skin is removed and the area is carefully closed with sutures