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Injection Procedure Clean the injection site with an alcohol swab and allow complete drying

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Abbreviations GC: Gastric cancer GSH: Glutathione ICIs: Immune checkpoint inhibitors ROS: Reactive oxygen species TCM: Central memory CD8+ T cells TEM: Effector memory CD8+ T cells TME: Tumor microenvironment References Sung H et al (2021) Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries

For this purpose, RAW 264.7 cells, a macrophage cell line obtained from a male mouse tumor induced with Abelson murine leukemia virus, were plated in complete DMEM (Euroclone) supplemented with 10% FBS in 48-well plates at a density of 2 10 5 cells per well