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SKU: 88436421914 · From vanierexcavation.com

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Description

Sadre-Marandi F, Dahdoul T, Reed MC, Nijhout HF

Gavett et al., 2011)

Its like a traffic jam at that critical biochemical intersection

Cullin4A-RBX1-DDB1 complex (GST-Cullin4A, TSF-RBX1 and TSF-DDB1), neddylated Cullin4A-RBX1-DDB1 complex (Uba3, UBC12, APPBP1, HA-NEDD8, GST-Cullin4A, TSF-RBX1 and TSF-DDB1), COP1-c-Jun complex (GST-COP1-TSF and MBP-c-Jun), COP1-DET1-c-Jun complex (GST-COP1, TSF-DET1 and MBP-c-Jun) were co-transfected in Expi293F cells and purified in the same procedures as described above

once those bonds are formed AP folds around them, stabilizing them against potential reductants during continued culture, cell harvesting, and extract preparation (Akiyama and Ito, 1993)