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These peptide-based hydroxylation assays demonstrating prolyl hydroxylation of IKK in a rabbit reticulocyte lysate system were not, however, consistently observed in a lysate-free decarboxylation assay [25]

Note : Always assess solvent compatibility during method validation to prevent adverse interactions with sensitive proteins

Trends Pharmacol Sci 38:592607 Prentice BM, Hart NJ, Phillips N, Haliyur R, Judd A, Armandala R, Spraggins JM, Lowe CL, Boyd KL, Stein RW, Wright CV, Norris JL, Powers AC, Brissova M, Caprioli RM (2019) Imaging mass spectrometry enables molecular profiling of mouse and human pancreatic tissue

Excess reactive oxidative species (ROS) in the follicular fluid can directly damage the egg