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After further 72 h in culture, cells were used for the SRB assay

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One study evaluating a high dose of alprostadil (40mcg/mL) vs papaverine 17.64mg, phentolamine 0.58mg, and alprostadil 5.8mcg/mL in patients who failed to respond to bi-mix found a much greater response rate in patients on tri-mix therapy (22% vs 50%) and a lower incidence of pain (41% vs 12.5%) in the tri-mix group as compared to the high dose alprostadil group