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However, stacking both requires a well-planned cycle, as IGF-1 is a potent growth factor that may increase systemic effects like muscle growth and insulin sensitivity

After washing, cells were stained with Alexa Fluor 488conjugated anti-rabbit (1:200, Invitrogen, A-10055) and Alexa Fluor 594conjugated anti-mouse (1:200, Invitrogen, A-21207) secondary antibodies for 1 h at RT

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