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The separated proteins were then transferred onto a PVDF membrane (Merck Millipore) and blocked using skim milk for 2 h at room temperature

The 28-day use window applies only to refrigerated storage

C., Tsai, H

This study received ethical committee approval from the Ethics Committee of the Second Hospital of Shandong University with written informed consent obtained from each participant prior to enrollment

Moreover, elevated AR activity may impair antioxidant defences through NADPH depletion, thereby limiting glutathione (GSH) regeneration and exacerbating oxidative stress 10,52 ( Figure 7 )