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RT-qPCR was carried out using the 2 Hieff UNICON Universal Blue qPCR Master Mix (Yeasen, Shanghai, China) under the following amplification conditions: Uracil-DNA Glycosylase (UDG) activation at 50 C for 2 min, initial denaturation at 95 C for 2 min, then 40 cycles of 95 C for 15 s and 60 C for 30 s for denaturation and annealing/extension, respectively, concluding with a melting curve analysis

doi: 10.3389/fpls.2017.00600

% in water, Sigma-Aldrich, #F8775) and HCl (0.5 mL, 36.538.0 %) were dissolved in 2 mL of dioxane (Sintorgan, #SIN-083003-63)

Modulating effects of a novel skin-lightening agent, alpha-lipoic acid derivative, on melanine production by the formation of DOPA conjugate products