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The intracellular metabolic adjustment is neutralized by the overexpression of a monocarboxylate transporter (MCT), resulting in a H + -linked co-transport of lactic acid across the plasma membrane, with increased extracellular acidity, known as lactic acidosis (Figure 1)

Quintana DS, Rokicki J, van der Meer D, Alnaes D, Kaufmann T, Cordova-Palomera A, et al

TCA was removed with organic solvents (three volumes of 1,1,2-trichloro-1,2,2-trifluroethane: one volume of trioctylamine) in a ratio of two volumes of organic solvent to one volume of sample

Results typically begin within 2472 hours, improving steadily over several days

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