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Pavlik, T

Briefly, both enzymatic and non-enzymatic crosslinks affect the collagen structure, altering both tissue biomechanics ( e.g ., altered tissue elastic modulus, ultimate tensile strength, stress relaxation, etc .) and failure mechanisms ( e.g ., crosslink breakage, load transfer, etc .)

The overexpressed fusion protein, which contains a GST tail, may be purified from the bacterial lysate in a single step by affinity chromatography on glutathione agarose