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In both HES lines, Grx1-roGFP2-HES2 and mito-Grx1-roGFP2-HES2, the sensor reacted in a concentration dependent manner to oxidation by H 2 O 2 with an increase in fluorescence ratio (R/R 0 ) under 405 nm excitation over 488 nm excitation (Figure 2E,F)

Additionally, a heat treatment (heating at 55C for 5 min) was performed with variants that were sufficiently stable, to induce precipitation of apo enzyme (Smith et al., 2023)

39 DollD

In contrast, [U- 13 C]glucose isotope tracing results demonstrated a significant increase in TCA cycle intermediates derived from glucose when GGCT was overexpressed

Data were fitted by linear regression