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Over the last 20 years, there have been significant efforts directed at understanding the etiology and the therapeutic challenges of this disease

A total of 200 Hy-Line Brown laying hens, 85 weeks old, were randomly distributed into two treatments, with 20 repetitions each, and five animals per repetition during 10 experimental weeks

The deficiency can occur when people have difficulty absorbing foods with B12 in the small intestine

To isolate mitochondria, cells were lysed in hypotonic lysis buffer (20 mM Tris buffer pH 7.4, 1 mM EDTA with phosphatase and protease inhibitors (Pierce protease and phosphatase inhibitor tablets)) and incubated on ice for 30 min

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