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For example: If you go to bed at 2am with a blood alcohol level of 0.20 and head to work at 9am it is likely that your blood alcohol level will be around 0.095 making you legally intoxicated

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Monolayers were permeabilized with 75% acetone in PBS for 3 min at room temperature, washed three times with PBS and incubated for 30 min at room temperature with the blocking solution (1% BSA in PBS)