hinnao glutathione – the anti-ageing molecule Hinnao Drops: the mother of

Proceedings of the National Academy of Sciences of the United States of America, 108 (19), 7763-7768

Glutathione in Mammalian Biology

Forehead Lines: Smooth horizontal lines across the forehead

Methods Reagents and antibodies Anti-Acsl4 antibody (ab155282, abcam), Anti-Fsp1 antibody (68049-1-Ig, Proteintech), Anti-Keap1 antibody (ab227828, Abcam), Anti-Gpx4 antibody (67763-1-Ig, Proteintech), Anti-F4/80 antibody (ab6640, Abcam), Anti- Ly6G antibody (ab25377, Abcam), Anti-Megalin antibody (ab184676, Aabcam), Anti-4-HNE antibody (ab48506, Abcam), Anti-GAPDH antibody (60004-1, Proteintech), BODIPY 581/591 C11 lipid peroxidation probe (D3861, Invitrogen), Mito-Tracker Red CMXRos fluorescent probe (C1035, Beyotime Biotechnology)

1 H NMR (400 MHz, DMSO- d 6 ) 12.30 (s, 1H), 10.99 (s, 1H), 7.67 (d, J = 7.2 Hz, 1H), 7.607.52 (m, 2H), 7.40 (d, J = 8.0 Hz, 1H), 6.766.68 (m, 1H), 5.13 (d, J = 13.1 Hz, 1H), 4.404.33 (m, 2H), 4.23 (dd, J = 17.4, 5.8 Hz, 1H), 4.06 (s, 2H), 3.83 (s, 1H), 3.27 (d, J = 6.6 Hz, 2H), 3.00 (t, J = 11.8 Hz, 1H), 2.952.86 (m, 1H), 2.73 (dd, J = 14.2, 8.3 Hz, 3H), 2.642.57 (m, 2H), 2.46 (s, 1H), 2.071.97 (m, 1H), 1.80 (d, J = 12.7 Hz, 2H), 1.621.49 (m, 1H), 1.42 (d, J = 12.7 Hz, 1H), 1.18 (d, J = 4.6 Hz, 9H)