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Following drug exposure time of 4 or 10 days, cells were washed with PBS, fixed with ice-cold methanol for 30 min at 4 C and stained with crystal violet, as published 56

In box plots, center lines denote medians, box limits indicate 25th/75th percentiles, and whiskers extend to the minimum and maximum values within 1.5 interquartile range, outliers beyond the whiskers are not shown

The processed sensorgrams were globally fitted to a 1:1 Langmuir binding model using the Biacore X100 Evaluation Software

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As research in this field continues to evolve, microbiome-based interventions hold promise for improving the treatment and prevention of gastrointestinal diseases