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Baoan ZhangHongwei Zhang, Xuehua Zhang, Liankai Zhang

After treatment with HRP blocker for 15 min and washing with wash buffer for 2 min, nuclear staining was performed with DAPI, and the slides were mounted with fluorescence mounting medium

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Lastly, STING can be taken up by non-canonical RAB22A-induced autophagosomes that do not fuse with lysosomes but are secreted to surrounding cells, spreading active STING