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Description

Vitamin B12 injections are typically used to treat a deficiency in people who are unable to absorb adequate amounts of B12

Immunocytochemistry and immunohistochemistry Glioblastoma patient specimens were obtained and analyzed in accordance with an Institutional Review Board-approved project plan (Kantonale Ethikkommission Zrich, Switzerland, KEK-ZH-Nr./BASCE-Nr

GBM cells were cultured at 37 C and 5% CO 2 in Neurobasal medium (ThermoFisher) supplemented with 1x B27 without vitamin A (Invitrogen), 10 ng/ml recombinant human EGF (R&D systems), 10 ng/ml basic FGF (Bio-Connect), 1x L-glutamine (VWR), indicated further as complete NB medium [43, 44]