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However, the activity reported was very low, which could be enhanced to 963-fold for a triple mutant E423Y/E442Q/D445N of BsGGT, developed by site-directed and random mutagenesis approach (Suzuki et al., 2010)

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Rhizomes Indian J Pharm Sci

Blood Cancer 62 , 17091716 (2015)

The working solution was prepared according to the instructions, and the serum was mixed with other reagents in the 96-well plate, then incubated at room temperature for 40 min, and the OD value was detected at the corresponding absorbance