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Although this study focused on methodological validation using isolated and purified clinical strains, the demonstrated high sensitivity, broad detection range, and robust anti-interference performance suggest potential for application to complex clinical samples like whole blood

Erythrocytes containing a small amount of reduced glutathione are relatively easily destroyed

Pernicious anaemia is a megaloblastic anaemia, because a lack of vitamin B12 also commonly referred to as cobalamin can cause abnormality in haematopoietic cell maturation[2]

Koch & Reimer, 2012)

Inadequate B12 levels impair: High-Risk Groups for Vitamin B12 Deficiency Vegetarians and Vegans Plant-based diets naturally contain minimal vitamin B12, as this nutrient occurs primarily in animal products