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The uncontrolled use of antibiotics in agriculture and inappropriate therapeutic practices has provided an evolutionary advantage to bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus (VRE), and others that have become resistant to one or multiple types of antibiotics, contributing to the dramatic situation in healthcare (Figure 2)

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Then the pellets were lysed by a lysis buffer