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For example, Gkountela S et al performed proteinase K digestion and bisulfite treatment of individual CTC cells or clusters with Zymo EZ DNA Methylation Direct Kit (Cat# D5020), generated a library with Illumina TruSeq DNA methylation kit (Cat# EGMK91396), amplified the library with Illumina Failsafe Enzyme (Cat# FSE51100), introduced indexes with Illumina Index Primers Kit (Cat# EGIDX81312), purified the library with Agencourt AMPure XP beads, and estimated the library concentration with Invitrogen Qubit DS DNA HS Assay Kit (Cat#Q32854) [120]

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