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The PCR was run for 40 cycles and the thermal cycling conditions were as follows: initial heat activation at 95 C for 10 minutes

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BBR was described as a potential antitumor agent that induces cell cycle arrest in G0/G1 phase, increases Cip/p21 and Kip/p27 protein expression, decreases expression of cyclin D1, D2, and DE, and the cyclin-dependent kinases Cdk2, Cdk4, and Cdk6, promoting apoptosis in HL-60 human leukemia cells (Li et al., 2017)

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verify the sequence on the certificate of analysis (CoA) from a third-party lab