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On one surface they are separated from the sinusoidal wall by a peri-sinusoidal space, the space of Disse, where they are exposed to tissue fluids

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10 l of CCK-8 solution (Cell Counting Kit-8, SAB Biotech, USA) was added to each well of the 96-well plate for 4 consecutive days to ensure even distribution of CCK-8 solution in each well

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