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(2023) demonstrated that C18-ceramide promoted mitophagy through LC3B lipidation to form LC3B-II

Scaffolds were incubated with primary antibodies overnight at 4 C and with secondary antibodies for 2 h at room temperature

These metabolic states appear to be related to the divergent functions of FoB and MZB, which have been documented in numerous studies 7,8,9,10,11,12,13,14

To clarify whether the first N-terminal residues of BNIP3 represent the discrete structural unit that directly can activate BAX, we conducted an established in vitro BAX activity assay utilising BNIP3-peptide 1-49, other BH3-only BAX activators (BIM and PUMA) as positive controls 38 , and bovine serum albumin and the antiapoptotic BCL XL as negative controls 2

End-stage tumors were harvested and tumor cells isolated and cultured in basal media (BFP), containing DMEM (Gibco 11965092) with 0.5% FBS (Gibco 16000044), antibiotic-antimycotic (Thermo Scientific 15240096), N2 supplement (Thermo Fisher Scientific, 17502-048), and 10 ng/ml each of recombinant human PDGF-AA (Peprotech, 100-13 A) and FGFb (Peprotech, 10018B50UG)