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doi: 10.3389/fcimb.2024.1339750 56 FanY.ChenX.ShanT.WangN.HanQ.RenB.et al

After centrifugation at 15,000 g at 4 C for 15 min, the supernatant was collected with 30 l used for input controls, and the remaining samples were incubated with the indicated antibody-coupled affinity beads for 2 h with gentle shaking at 4 C